RAPID DOA PANEL TEST

For the qualitative assessment of drugs and their metabolites in human urine.

INTENDED USE

All of Rapid DOA Panel Test is an immunochromatography based one step in vitro test. It is designed for qualitative determination of drug substances in human urine specimens. This assay may be used in the point of care setting.

PROCEDURE

1)        Bring all materials and specimens to room temperature.

2)        Remove the test card from sealed foil pouch.

3)        Place the sample pad end into the urine specimen being careful to hold each pad in the urine without touching the plastic card.

4)        Hold the card in the urine for 10 seconds, remove from the urine and replace the cap.

5)        Read the results at 5 minutes after adding the sample.

Do not interpret the result after 5 minutes.

PRINCIPLE

Each component strip of Rapid DOA Panel is based on the principle of specific immunochemical reaction between antibodies and antigen to analyze particular compound in human urine specimen. The assay relies on the competition for binding antibody. When drug is present in the urine specimen, it competes with drug conjugate for the limited amount of antibody-dye conjugate. When the amount of drug is equal or more than the cut-off, it will prevent the binding of drug conjugate to the antibody. Therefore, a positive urine specimen will not show a colored band on the test line zone, indicating a positive result, while the presence of a colored band indicates a negative result.

A control line is present in the test window to work as procedural control. This colored band should always appear on the control line zone if the test device is stored in good condition and the test is performed appropriately.